effects of diode and he-ne laser on in vitro production of anthocyanin in apple cell suspension culture
نویسندگان
چکیده
plant cell cultures could be used as an important tool for biochemical production, ranging from natural pigments to pharmaceutical products. anthocyanin is affected by a variety of factors. light, an important plant environmental factor, influences the anthocyanin production in vegetative plant tissues. here, we have investigated the influence of the blue laser-diode (bld) and red laser-he ne (rlhn) irradiation on the ability of apple cells in producing anthocyanin in suspension culture. callus was induced from young leaf of apple and cell suspension cultures were subsequently formed from calluses. the normal cell suspension into l-shape tube test exposed to rlhn (6.46, 4.82, 1.54 mwcm-2 and 666.66 μwcm-2) and bld(67.09, 32.74, 30.4, 13.73 mwcm-2) laser radiation for 20 min. results showed that theanthocyanin production was enhanced significantly by both rlhn and brd compared withdarkness and fluorescent light cells (p
منابع مشابه
Effects of Diode and He-Ne Laser on In Vitro Production of Anthocyanin in Apple Cell Suspension Culture
Plant cell cultures could be used as an important tool for biochemical production, ranging from natural pigments to pharmaceutical products. Anthocyanin is affected by a variety of factors. Light, an important plant environmental factor, influences the anthocyanin production in vegetative plant tissues. Here, we have investigated the influence of the blue laser-diode (BLD) and red laser-He Ne (...
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Light is an effective factor in cell suspension culture and must be controlled for optimizing cell growth. Growth of anthocyanin producing suspension cells of a red-fleshed genotype of apple (RFA) was assessed in response to blue diode laser (BDL) and red He Ne (RHNL) laser. The suspension cells in L-shaped test tubes were exposed to short-term laser radiation for 20 min in a rotary shaker. The...
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The red-fleshed apples are from varieties of apples confer the competence and high ability in vitro to produce anthocyanins in their organs. Several factors affect the in vitro production of anthocyanins in plant tissue culture and to produce high, the optimizing of these factors are very necessary. Light is one of the most important factors that many studies have been done in this area but the...
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عنوان ژورنال:
international journal of horticultural science and technologyناشر: iranian society for horticultural science university of tehran
ISSN 2322-1461
دوره 2
شماره 2 2015
کلمات کلیدی
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